Bacterial expression ready vector to protein

From bacterial expression ready vector(customer provided) to protein

CPA002: $599

1.     Antigen

Customer provided bacterial expression ready vactor with protocol if any.

2.     Protein purify and conjugation

Sunny will express and purify antigen protein and 10 mg of them will be conjugated with 5 mg of KLH or BSA carrier protein for immunization and screening.

3.    Rabbit immunization and Antiserum purification

We offer optimized 56-day immunization protocol with a very high success rate.  Antiserum purification will be carried out by Protein A or immunogen-specific affinity chromatography.  Usually, 2-3mg peptides, 1-2ml pre-immune sera, 20 ml of serum and 2-5mg purified antiserum will be provided to customer.

4.    Report

       We will provide ELISA.  With additional charges, we can assist customer to  characterize the purified antibody using a variety of assays including Western bloting (WB), immunohistochemistry (IHC), immunofluoresence (IF) and flow cytometry (FACS).

       Please provide: PI Name, Phone number, Email and  Mailing Address in Add Comments About Your Order during checkout and download Purchase Order Form and Fax or Email to us.